OD-specific total cell volume when grown on complex medium and different carbon sources

Range 2.4 to 4.5 Table - link μl/ml/OD
Organism Bacteria Escherichia coli
Reference Volkmer B, Heinemann M (2011) Condition-Dependent Cell Volume and Concentration of Escherichia coli to Facilitate Data Conversion for Systems Biology Modeling. PLoS ONE 6(7): e23126. doi:10.1371/journal.pone.0023126 p.2 table 1PubMed ID21829590
Method When multiplying the cell volume (Figure 1A BNID 106577) and the OD-specific cell concentration (cells/ml/OD Figure 1B, BNID 106578) one can obtain an OD-dependent total volume of the cells (µl/ml/OD Figure 2A).
Comments P.2 right column bottom paragraph: "Consistent with the literature, the length of the cells varied with the condition, between 1.6 µm for stationary cells and 3.9 µm for cells growing on LB medium (Table 1). Since E. coli cells grow by elongation, the cell length of individuals varies greatly in a population, thereby leading to a large standard deviation of the average cell length. Also in agreement with the literature, [researchers] found the cell width to be condition-independent (1.26 µm ±0.16 µm)." Table also has data for cells grown under stress conditions and different dilution rates. E. coli K-12 strain BW25113. Tests with the strain MG1655 on a few selected conditions revealed comparable results. Here, OD was measured at 600 nm. Note: OD readings might vary between different spectrophotometers.
Entered by Uri M
ID 106579