Cell concentration for culture at stationary phase with OD600 of 1 at 10°C

Value 9e+6 Cells/ml
Organism Budding yeast Saccharomyces cerevisiae
Reference Margesin R. Effect of temperature on growth parameters of psychrophilic bacteria and yeasts. Extremophiles. 2009 Mar13(2):257-62 p.261 fig.2 right bottom graphPubMed ID19057843
Method P.258 right column 2nd paragraph: "Culture turbidity (optical density) was measured spectrophotometrically a 600 nm (OD600). Numbers of viable cells were determined by the plate count method on the bacterial or yeast medium as described above, solidified with agar (15 g/l). Colony-forming units (cfu) were counted after 2 days at 25°C (Pedobacter heparinus, Saccharomyces cerevisiae), 3 days at 20°C (Pedobacter piscium, P.heparinus, Leucosporidiella creatinivora), or after 5 days at 15°C (Rhodotorula glacialis). Only plates containing 30–300 colonies were used for statistically valid enumeration (Koch 1994)." Value extracted visually from fig. 2
Comments
Entered by Uri M
ID 106299