||Bacteria Escherichia coli
||Wang HH et al., Programming cells by multiplex genome engineering and accelerated evolution. Nature. 2009 Aug 13 460(7257):894-8. p.897 right column top paragraph and p.895 caption of figure 4PubMed ID19633652
||p.897 right column top paragraph: "Allelic replacement: Liquid cell cultures were inoculated from colonies obtained from a plate and grown at 30°C to an absorbance (600 nm) of ~0.7
(~7×10^8 cells/ml) in a rotor drum at 200 r.p.m."