Doubling time of E. coli BL21 (DE3) aerobic batch culture in LR medium supplemented with 0.4% glycerol

Value 66 min Range: ±3 Table - link min
Organism Bacteria Escherichia coli
Reference Gunasekera TS, Paliy O. Growth of E. coli BL21 in minimal media with different gluconeogenic carbon sources and salt contents. Appl Microbiol Biotechnol. 2007 Jan73(5):1169-72. p.1171 Table 1PubMed ID16944129
Method P.1170 right column top paragraph: "Growth was monitored spectrophotometrically by measuring each culture’s OD600nm periodically." P.1171 left column bottom paragraph: "[Investigators] also examined the aerobic growth of BL21 in different minimal media. Three different minimal media were used: M9 minimal medium, commonly used in microbiological studies N−C− minimal medium, utilized for physiological experiments and gene expression studies (Soupene et al. 2003 Gyaneshwar et al. 2005) and LR medium, used for preparation of deuterated components in E. coli (LeMaster and Richards 1982 Paliy et al. 2003)."
Comments
Entered by Uri M
ID 105397