Peak apoptotic response after spinal chord injury

Value 15 Hours
Organism Teleost fish Apteronotus leptorhynchus
Reference Sîrbulescu RF, Ilies I, Zupanc GK. Structural and functional regeneration after spinal cord injury in the weakly electric teleost fish, Apteronotus leptorhynchus. J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2009 Jul195(7):699-714PubMed ID19430939
Method To determine the spatiotemporal pattern of apoptosis after SCI, researchers used active caspase-3, an executioner caspase, as a marker of apoptosis. Transection lesions of the spinal cord were applied by amputating 1-cm caudal segments of the tail, and were followed by post-amputation survival times of 0–50 days. A highly significant change in the number of apoptotic cells was observed [F(12,26) = 7.126, P < 0.001, one-way ANOVA].
Comments An increase in the number of caspase-3- positive cells, relative to baseline levels, was observed around 12 h of survival, reaching maximum levels after 15 h (P < 0.05, Duncan post-hoc test Fig. 3a–f, h). After 24 h post-lesion, the apoptotic cells declined in number, first sharply until 5 days of survival, then more slowly until baseline levels were reached around 30 days post amputation (Fig. 3 h). Teleost fish and urodele amphibians (salammanders) differ markedly from mammals in their much higher ability to recover from spinal chord and brain injury.
Entered by Uri M
ID 104869