| Range |
~5 mM
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Shabala L, Bowman J, Brown J, Ross T, McMeekin T, Shabala S. Ion transport and osmotic adjustment in Escherichia coli in response to ionic and non-ionic osmotica. Environ Microbiol. 2009 Jan11(1):137-48. doi: 10.1111/j.1462-2920.2008.01748.x. p.141 fig.3PubMed ID18793315
|
| Method |
"non-invasive microelectrode ion flux (MIFE) measurement technique." "Escherichia coli Frag1 was maintained on Luria–Bertani agar. Culture was grown in side-arm flasks containing glucose minimal media (DM, Minimal Broth Davis, Difco) supplemented with 0.1% of glucose at 25°C with shaking (60 r.p.m.) until cells reached exponential growth phase (optical density OD540 = 0.3). The culture was used for the MIFE experiments, intracellular measurements and MP measurements." "Exponential phase cells were treated with different concentrations of NaCl (ranging between 1% and 10% with 1% increment 0.3–3.53 Os kg-1 range) and isotonic concentrations of sucrose for 1 h." Extracted visually from fig.3 |
| Comments |
"Changes in internal Na+ content essentially mirrored those for K+ content, with a strong negative correlation (r=-0.94) between intracellular K+ and Na+ found in response to NaCl treatment (Fig. 3). The critical concentration appears to be 5% NaCl, after which the intracellular Na+ increased almost 10-fold, to the level of above 1 M (note the logarithmic scale in Fig. 3). Interestingly, at exactly that concentration (5%) the intracellular K+ level dropped below control values, as evident from Fig. 3. At the same time, no significant changes in internal Na+ content occurred in response to sucrose treatments for the entire range of concentrations tested." Under range of 0-3.5 Os/Kg external osmolality of sucrose in medium. |
| Entered by |
Uri M |
| ID |
104050 |