Genome size measured by flow-cytometry
Please note: The entry will be shown to all once approved by the database administrator.
| Value | 1.57e+8 base pairs |
|---|---|
| Organism | Thale cress Arabidopsis thaliana |
| Reference | Bennett MD, Leitch IJ, Price HJ, Johnston JS. Comparisons with Caenorhabditis (approximately 100 Mb) and Drosophila (approximately 175 Mb) using flow cytometry show genome size in Arabidopsis to be approximately 157 Mb and thus approximately 25% larger than the Arabidopsis genome initiative estimate of approximately 125 Mb. Ann Bot (Lond). 2003 Apr91(5):547-57.PubMed ID12646499 |
| Method | Flow cytometry, a method that can obtain multiparametric measurements of thousands of microscopic particles such as cells or chromosomes suspended in a stream of fluid per second. When C. elegans and arabidopsis were co-run in mixtures, the 2C peak for arabidopsis averaged approx. 57% above that for C. elegans (relative fluorescence of nuclear samples stained with propidium iodide (PI), Fig. 1D), giving a 1C DNA amount of approx. 157 Mb for arabidopsis based on an estimate of 100 Mb for C. elegans (p. 550 left column). |
| Comments | Complete sequencing of the arabidopsis genome until no gaps remain at telomeres, NORs or centromeres, as originally intended, is still needed. Not only would this provide a precise angiosperm C-value as an invaluable benchmark calibration standard for other plants, but it would also ensure that no important genes have been missed, especially in centromeric gap regions which are clearly larger than previously imagined. See BNID 105472 for original genome size estimate of 125Mb. |
| Entered by | Uri M |
| ID | 104000 |