Number of mRNA transcripts per cell
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| Value | 60000 copies/cell |
|---|---|
| Organism | Budding yeast Saccharomyces cerevisiae |
| Reference | Zenklusen D, Larson DR, Singer RH. Single-RNA counting reveals alternative modes of gene expression in yeast.Nat Struct Mol Biol. 2008 Dec15(12):1263-71.PubMed ID19011635 |
| Method | an in situ hybridization (FISH) approach that detects single mRNA molecules, mRNA abundance and transcriptional activity within single Saccharomyces cerevisiae cells was measured. See fig. 8a: Synthesis time was plotted against the length of the gene. The slope of the line gives (polymerase speed)^-1 |
| Comments | The genes used in this study show a 3 to 6 fold higher expression than that determined previously (Holstege et al, Cell 1998 pubmed 9845373, report a value of 15000 mRNA transcripts per cell). See BNID 104312 |
| Entered by | Uri M |
| ID | 103023 |