| Comments |
P.1 right column top paragraph: "Thus, labeling of bacterial RNAs requires either direct labeling of total RNA or the use of a cDNA synthesis process in which a pool of gene-specific or random oligonucleotide primers replace the oligo(dT) primers (refs 9,13). Neither sample preparation method enriches the labeled target for mRNA. Thus, much of the label is incorporated into rRNAs and tRNAs, which make up 95–97% of the total RNA isolated from bacterial cells." See Sørensen et al., 2017 PMID 29023189 p.6 top paragraph: "Since tRNA and rRNA together make up more than 95% of the
total RNA, (refs 26, 27, in E. coli)..." |