Number of cells transformed by single bombardment with DNA coated tungsten particle

Range 2-20 cells/bombardment
Organism Plants
Reference Svab Z, Maliga P. High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene. Proc Natl Acad Sci U S A. 1993 Feb 190(3):913-7.PubMed ID8381537
Primary Source Kanevski, I. F., Thakur, S., Cosowsky, L., Sunter, G., Brough, C., Bisaro, D. & Maliga, P. (1992) Tobacco lines with high copy number of replicating recombinant geminivirus vectors after biolistic DNA delivery. Plant J. 2, pp. 459 Table 1
Method Tobacco suspension culture cells were bombarded with the recombinant DNA A constructs and selected for kanamycin resistance.
Entered by Uri M
ID 102762