Kcat of beta Carbonic Anhydrase

Value 1e+6 1/sec
Organism Pea Pisum sativum
Reference Lindskog S. Structure and mechanism of carbonic anhydrase. Pharmacol Ther. 1997 74(1):pp. 2PubMed ID9336012
Primary Source Johansson IM, Forsman C. Solvent hydrogen isotope effects and anion inhibition of CO2 hydration catalysed by carbonic anhydrase from Pisum sativum. Eur J Biochem. 1994 Sep 15 224(3):901-7.PubMed ID7925414
Method cDNA encoding pea carbonic anhydrase was isolated and cloned into a mutagenesis expression vector giving the plasmid pPCA. Initial rates of CO2 hydration were measured in a hi-tech stopped-flow spectrophotometer at 25 degrees c by the changing pH-indicator method
Comments A Kcat value of 10^6 1/sec was calculated for the subunit of the pea enzyme upon extrapolation to infinite concentrations of barbital buffer
Entered by Uri M
ID 102672