| Range |
0.09-0.22 Table - link unitless
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Bremer, H., Dennis, P. P. (1996) Modulation of chemical composition and other parameters of the cell by growth rate. Neidhardt, et al. eds. Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology, 2nd ed. chapter 97 Table 1 |
| Primary Source |
Dennis PP, Bremer H. Differential rate of ribosomal protein synthesis in Escherichia coli B/r. J Mol Biol. 1974 Apr 15 84(3):407-22. AND Dennis PP, Bremer H. Macromolecular composition during steady-state growth of Escherichia coli B-r. J Bacteriol. 1974 Jul119(1):270-81.PubMed ID4618855, 4600702
|
| Method |
"The differential rate of r-protein synthesis was calculated from the data in tables 1 [ link ] and 2 [ link ] and the relationship for ar (equation 6, Table 5: ar = (R/P)×[(aa/ribosome) ×fs ×(1–ft)/(nucl./rib)].)." R=RNA,P=Protein, fs=fraction of total RNA that is stable RNA, ft=fraction of stable RNA that is tRNA. Determined also from measurements of the protein content of ribosomes after labeling with radioactive leucine and uridine, see primary refs |
| Comments |
"A measure for the synthesis of r-protein is its proportion of total protein, ar. Values of ar have been determined (i) from the protein content of ribosomes and (ii) from the RNA-to protein ratio. Both methods give essentially identical values. The proportion of r-protein increases with growth rate from 9% at 0.6 to 21% at 2.5 doublings per h. It has been reported that the r-protein mRNA synthesis per total RNA synthesis rate is nearly invariant with growth rate, but, when expressed as a fraction of the rate of (total) mRNA synthesis, it increases with growth rate like ar or rRNA synthesis (Fig. 1 refs 42, 63, 89). These observations support the idea that, to a first approximation, r-protein production is matched to rRNA production at the level of transcription." For values at different growth rates see table 3 Table - link . See BNID 106422 |
| Entered by |
Uri M |
| ID |
102345 |