cell concentration for culture with OD600 of 1
Please note: The entry will be shown to all once approved by the database administrator.
| Value | 3e+7 cell/ml |
|---|---|
| Organism | Budding yeast Saccharomyces cerevisiae |
| Reference | Audra Day, Colette Schneider, and Brandt L. Schneider,chapter 6- Yeast Cell Synchronization, Methods Mol Biol. 2004 241:55-76.PubMed ID14970646 |
| Primary Source | Short protocols in molecular biology, Fred Ausubel et al., 5th ed. Vol. 2 pp. 13-9 |
| Comments | Best to measure at OD<1 to insure linearity. Value depends on strain and conditions to a factor of several fold. Cell density is most accurately determined with a Z2 Coulter Counter Channelyzer. However, a spectrophotometer and OD600 absorbance readings can be substituted. One OD600 is equivalent to approx 3 × 107 cells/mL (3). However, OD600 absorbance readings are sensitive to the size of cells. In block-and-release protocols, where cell size increases dramatically, OD600 absorbance readings will increase whereas the cell number does not. |
| Entered by | Ron Milo - Admin |
| ID | 100986 |