||Bacteria Escherichia coli
||Zhu J, Penczek PA, Schröder R, Frank J. Three-dimensional reconstruction with contrast transfer function correction from energy-filtered cryoelectron micrographs: procedure and application to the 70S Escherichia coli ribosome. J Struct Biol. 1997 Apr118(3):197-219 pp.212-213 figs.8-9PubMed ID9169230
||Cryoelectron microscopy provides the means of studying macromolecules in their native state. However, the contrast transfer function (CTF) makes the images and the three-dimensional (3D) maps derived from them difficult to interpret. Resarchers developed methods to determine the CTF from experimental data and to obtain a CTF-corrected 3D reconstruction. The CTF correction and 3D reconstruction accomplished in one step made it easy to combine different defocus data sets and decrease the error accumulation in the computation.
||Value measured visualy from figures 8 & 9 depicting reconstructed ribosome images. Scale bar is 10 nm and diameter was measured to be ~26nm. See BNID 102320 for value of 21 nm
||Ron Milo, Paul Jorgensen, Mike Springer