Diameter of 70S (intact) ribosome

Value ~26 nm
Organism Bacteria Escherichia coli
Reference Zhu J, Penczek PA, Schröder R, Frank J. Three-dimensional reconstruction with contrast transfer function correction from energy-filtered cryoelectron micrographs: procedure and application to the 70S Escherichia coli ribosome. J Struct Biol. 1997 Apr118(3):197-219 pp.212-213 figs.8-9PubMed ID9169230
Method Cryoelectron microscopy provides the means of studying macromolecules in their native state. However, the contrast transfer function (CTF) makes the images and the three-dimensional (3D) maps derived from them difficult to interpret. Resarchers developed methods to determine the CTF from experimental data and to obtain a CTF-corrected 3D reconstruction. The CTF correction and 3D reconstruction accomplished in one step made it easy to combine different defocus data sets and decrease the error accumulation in the computation.
Comments Value measured visualy from figures 8 & 9 depicting reconstructed ribosome images. Scale bar is 10 nm and diameter was measured to be ~26nm. See BNID 102320 for value of 21 nm
Entered by Ron Milo, Paul Jorgensen, Mike Springer
ID 100121