| Range |
girth 7–9 µm: peak average velocity >20 µm/min
|
| Organism |
Mouse Mus musculus |
| Reference |
jacobelli et al., Confinement-optimized three-dimensional T cell amoeboid motility is modulated via myosin IIA-regulated adhesions. Nat Immunol. 2010 Oct11(10):953-61. doi: 10.1038/ni.1936. p.957 left column top paragraph & P.960 left column 2nd paragraphPubMed ID20835229
|
| Method |
p.956 right column bottom paragraph:"First [investigators] analyzed whether the degree of confinement affects T cell motility. For these experiments [they] used microchannels with a fixed ceiling height of 7µm, a dimension similar to the girth of an activated T cell. [They] also determined how different adhesiveness of the substrate may influence confined T cell migration by coating the microchannels with fibronectin, ICAM-1 or casein (which is not an integrin ligand and blocks integrin ligand binding to the substrate) (Fig. 5c–e). In all cases, [they] observed peak velocities at optimal confinement and reduced speeds when cells were too tightly or too loosely enclosed." |
| Comments |
P.957 left column top paragraph:"In microchannels with widths that approximate their girth (7–9 µm), T cells migrated at peak average velocities that exceeded 20 µm/min in the presence of the integrin substrates fibronectin and ICAM-1 (Fig. 5c,d)." P.960 left column 2nd paragraph:"Under ideal confinement, [investigators] observed migration speeds greater than 20µm/min these are higher than average speeds but equivalent to the fastest instantaneous speeds reported for motility in lymph nodes. This could be due to the fact that individual microchannels provide a consistent and in some cases optimized (i.e. ~8µm channels) environment for T cell migration. In contrast, within lymph nodes, lymphocytes likely encounter varying microenvironments with different characteristics which may modulate motility speeds and limit peak velocities." |
| Entered by |
Uri M |
| ID |
112246 |