mCherry maturation half-time

Range ~40 min
Organism Budding yeast Saccharomyces cerevisiae
Reference Khmelinskii A et al., Tandem fluorescent protein timers for in vivo analysis of protein dynamics. Nat Biotechnol. 2012 Jun 24 30(7):708-14. doi: 10.1038/nbt.2281. p.709 left column 3rd paragraphPubMed ID22729030
Primary Source Merzlyak EM et al., Bright monomeric red fluorescent protein with an extended fluorescence lifetime. Nat Methods. 2007 Jul4(7):555-7.PubMed ID17572680
Comments "...[investigators] combined the monomeric red fluorescent protein mCherry, which matures with a half-time of ~40 min [primary source], with a monomeric variant of the superfolder green fluorescent protein sfGFP, which becomes fluorescent within minutes of synthesis [ref 18]. A pool of mCherry-sfGFP molecules should be mostly green-fluorescent shortly after synthesis and gradually acquire red fluorescence over time, such that the ratio of red to green fluorescence is a function of the age of the protein pool (Fig. 1b)."
Entered by Uri M
ID 110551