Fraction of essential proteins that can accommodate a tag at the C- terminus (without loss of function)

Please note: The entry will be shown to all once approved by the database administrator.
Range ~84 %
Organism Budding yeast Saccharomyces cerevisiae
Reference Khmelinskii A et al., Tandem fluorescent protein timers for in vivo analysis of protein dynamics. Nat Biotechnol. 2012 Jun 24 30(7):708-14. doi: 10.1038/nbt.2281. Supporting Online Material p.S8 3rd paragraphPubMed ID22729030
Primary Source Huh WK, Falvo JV, Gerke LC, Carroll AS, Howson RW, Weissman JS, O'Shea EK. Global analysis of protein localization in budding yeast. Nature. 2003 Oct 16 425(6959):686-91 & Ghaemmaghami, S. et al. Global analysis of protein expression in yeast. Nature. 2003. 425(6959) pp.737-41PubMed ID14562095, 14562106
Comments "C- terminal tagging – The experiments conducted in this study used synthetic constructs and endogenous yeast proteins tagged with mCherry - sfGFP mostly at the C - terminus. Although a tag can influence the marked protein, C - terminal tagging appears to have the lowest likelihood of interfering with protein function and ~84% of essential proteins (total of 1034 essential proteins) in S. cerevisiae can accommodate a tag at the C- terminus [primary sources]."
Entered by Uri M
ID 110547