Fraction of essential proteins that can accommodate a tag at the C- terminus (without loss of function)
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Range | ~84 % |
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Organism | Budding yeast Saccharomyces cerevisiae |
Reference | Khmelinskii A et al., Tandem fluorescent protein timers for in vivo analysis of protein dynamics. Nat Biotechnol. 2012 Jun 24 30(7):708-14. doi: 10.1038/nbt.2281. Supporting Online Material p.S8 3rd paragraphPubMed ID22729030 |
Primary Source | Huh WK, Falvo JV, Gerke LC, Carroll AS, Howson RW, Weissman JS, O'Shea EK. Global analysis of protein localization in budding yeast. Nature. 2003 Oct 16 425(6959):686-91 & Ghaemmaghami, S. et al. Global analysis of protein expression in yeast. Nature. 2003. 425(6959) pp.737-41PubMed ID14562095, 14562106 |
Comments | "C- terminal tagging – The experiments conducted in this study used synthetic constructs and endogenous yeast proteins tagged with mCherry - sfGFP mostly at the C - terminus. Although a tag can influence the marked protein, C - terminal tagging appears to have the lowest likelihood of interfering with protein function and ~84% of essential proteins (total of 1034 essential proteins) in S. cerevisiae can accommodate a tag at the C- terminus [primary sources]." |
Entered by | Uri M |
ID | 110547 |