Maturation time for sfGFP (super folded GFP)

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Value 5.63 min Range: ±0.82 min
Organism Budding yeast Saccharomyces cerevisiae
Reference Khmelinskii A et al., Tandem fluorescent protein timers for in vivo analysis of protein dynamics. Nat Biotechnol. 2012 Jun 24 30(7):708-14. doi: 10.1038/nbt.2281. Supporting Online Material p.S4 bottom paragraphPubMed ID22729030
Comments "Maturation rate constants were calculated independently for each cell. Subsequently, final maturation rate constants were determined as medians values (n=35), considering only the sub-population of cells yielding a better than median ?^2 value in model fitting. The obtained maturation half-time [is]... T=5.63±0.82min for sfGFP (median±s.e.m.) (Supplementary Fig. 8a)."
Entered by Uri M
ID 110546