Number of Bicoid molecules at cycle 14

Value 1.5e+8 molecules/cell Range: ±0.2х10^8 molecules/cell
Organism Fruit fly Drosophila melanogaster
Reference Drocco JA, Grimm O, Tank DW, Wieschaus E. Measurement and perturbation of morphogen lifetime: effects on gradient shape. Biophys J. 2011 Oct 19 101(8):1807-15. doi: 10.1016/j.bpj.2011.07.025. p.1813 left column 2nd paragraphPubMed ID22004733
Method P.1813 left column 2nd paragraph: "A typical Western blot is shown in Fig. 5 a in Fig. 5 b, the quantification of five different experiments that represent a total of 400 embryos per time point is plotted. [Researchers] calibrate the blot to known quantities of green fluorescent protein to determine the absolute number of Bcd molecules in the embryo."
Comments P.1813 left column 2nd paragraph: "The number of molecules continues to increase until cycle 14, at which time point [researchers] find a total of 1.5±0.2×10^8 Bcd molecules in the embryo."
Entered by Uri M
ID 109198