Number of Bicoid molecules at cycle 14

Value 1.5e+8 molecules/cell Range: ±0.2х10^8 molecules/cell
Organism Fruit fly Drosophila melanogaster
Reference Drocco JA, Grimm O, Tank DW, Wieschaus E. Measurement and perturbation of morphogen lifetime: effects on gradient shape. Biophys J. 2011 Oct 19 101(8):1807-15. doi: 10.1016/j.bpj.2011.07.025. p.1813 left column 2nd paragraphPubMed ID22004733
Method "A typical Western blot is shown in Fig. 5 a in Fig. 5 b, the quantification of five different experiments that represent a total of 400 embryos per time point is plotted. [Researchers] calibrate the blot to known quantities of green fluorescent protein to determine the absolute number of Bcd molecules in the embryo."
Comments "The number of molecules continues to increase until cycle 14, at which time point [researchers] find a total of 1.5±0.2?10^8 Bcd molecules in the embryo."
Entered by Uri M
ID 109198