| Value |
50
min
|
| Organism |
Fruit fly Drosophila melanogaster |
| Reference |
Drocco JA, Grimm O, Tank DW, Wieschaus E. Measurement and perturbation of morphogen lifetime: effects on gradient shape. Biophys J. 2011 Oct 19 101(8):1807-15. doi: 10.1016/j.bpj.2011.07.025. p.1813 left column top paragraphPubMed ID22004733
|
| Method |
"The conceptual idea in [researchers'] strategy to measure Bcd lifetime is illustrated in Fig. 1 f." |
| Comments |
"A[n] estimate for general cytoplasmic flux
between cycles 12 and 14 was obtained using live imaging
to follow the redistribution of the Histone-RFP. Correcting
for this flux has a minor effect on the degradation rate as a
function of developmental time (Fig. 4 e, red line) but yields
a degradation rate before cycle 14 of 0.020±006 min^-1,
and a lifetime estimate of 50 min, identical to that measured
at the beginning of cycle 14. [Researchers] conclude that from cycle
11 to the beginning of cycle 14, the degradation rate of
Bcd protein is approximately constant." |
| Entered by |
Uri M |
| ID |
109197 |