Transcription and translation rates

Range Table - link
Organism Bacteria Escherichia coli
Reference Proshkin S, Rahmouni AR, Mironov A, Nudler E. Cooperation between translating ribosomes and RNA polymerase in transcription elongation. Science. 2010 Apr 23 328(5977):504-8. p.505 table 1PubMed ID20413502
Method "To calculate the elongation rate, the Escherichia coli culture was induced with IPTG, and the time that elapsed between the appearance of a specific hybridization signal from probes complementary to the 5' and 3' segments of the lacZ transcript was determined by dotblot hybridization (Fig. 1 and fig. S1) (refs 5, 6)."
Comments "...[researchers] found that for most of the coding region, the first trailing ribosome directly assists RNAP during elongation. Such cooperation between the two macromolecules explains the precise match of translational and transcriptional rates under various growth conditions (Table 1)." See note above table
Entered by Uri M
ID 108487