Range |
Table - link
|
Organism |
Bacteria Escherichia coli |
Reference |
Proshkin S, Rahmouni AR, Mironov A, Nudler E. Cooperation between translating ribosomes and RNA polymerase in transcription elongation. Science. 2010 Apr 23 328(5977):504-8. p.505 table 1PubMed ID20413502
|
Method |
"To calculate the elongation rate, the
Escherichia coli culture was induced with IPTG,
and the time that elapsed between the appearance
of a specific hybridization signal from
probes complementary to the 5' and 3' segments
of the lacZ transcript was determined by dotblot
hybridization (Fig. 1 and fig. S1) (refs 5, 6)." |
Comments |
"...[researchers] found that for
most of the coding region, the first trailing ribosome
directly assists RNAP during elongation.
Such cooperation between the two macromolecules
explains the precise match of translational
and transcriptional rates under various growth
conditions (Table 1)." See note above table |
Entered by |
Uri M |
ID |
108487 |