| Value |
1000
Fold
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Winkler W, Nahvi A, Breaker RR. Thiamine derivatives bind messenger RNAs directly to regulate bacterial gene expression. Nature. 2002 Oct 31 419(6910):952-6.PubMed ID12410317
|
| Method |
Researchers prepared ß-galactosidase translational fusion constructs that encompass the 5'-untranslated regions of thiM and thiC mRNAs of E. coli, each of which includes a previously identified ‘thi box’ domain. The sensitivity of metabolite detection by these mRNAs was assessed by establishing apparent dissociation constant (apparent KD) values for TPP, thiamine, and thiamine monophosphate (TP). Values were generated by monitoring the extent of spontaneous cleavage at several ligand-sensitive sites within the RNA over a range of ligand concentrations. |
| Comments |
Both 165 thiM and 240 thiC RNAs (numbers refer to number of nucleotides in RNA) appear to bind TPP more readily than TP or thiamine, with thiC exhibiting more than 1,000-fold discrimination against TP and thiamine (see fig. 2b). |
| Entered by |
Uri M |
| ID |
105441 |