Kcat of human brain 6PF2K wild type
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| Value | 0.407 Sec^-1 Range: ±0.022 Sec^-1 |
|---|---|
| Organism | Human Homo sapiens |
| Reference | Manes NP, El-Maghrabi MR. The kinase activity of human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase is regulated via inhibition by phosphoenolpyruvate. Arch Biochem Biophys. 2005 Jun 15 438(2):125-36 Table - link PubMed ID15896703 |
| Method | Isolation of human brain 6PF2K/Fru-2,6-P2ase isoform cDNAs and gene. Subcloning and mutagenesis. Expression and purification of 6PF2K/Fru-2,6-P2ase. Protein kinase assay. For equations and method used to determine 6PF2K kinetic models and statistics see text. |
| Comments | The 6PF2K (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase) activity was measured at pH 7.5 with 5 mM ATP and 1–5000 µM Fru-6-P. The Fru-2,6-P2ase activity was measured at pH 7.5 with 50 µM Fru-2,6-P2. |
| Entered by | Uri M |
| ID | 104959 |