Highest cell densities by various propagation techniques of E. coli

Range Table - link
Organism Bacteria Escherichia coli
Reference Shiloach J, Fass R. Growing E. coli to high cell density a historical perspective on method development. Biotechnol Adv. 2005 Jul23(5):345-57PubMed ID15899573
Primary Source List of refs in table
Comments Note: this table gives value of 190 g/L. The cell density obtained by Gallup and Gerhardt (see table) is very close to the maximal possible density of an E. coli liquid culture, which today is estimated to be about 200 g/l dcw. According to Lee (1996), E. coli culture fluidity is lost when the dry cell weight is higher than 220 g/l. See BNID 104941 for highest value of 20.5 g/L on Luria Bertani broth. Note-The values in table (54-190 g/L) are reached when a limited amount of nutrients is supplied. Counter-intuitively, as nutrient levels in medium increase they become inhibitory and a lower cell density can be reached ~15g/L for the maximum non-inhibitive concentration of nutrients (BNID 105323).
Entered by Uri M
ID 104942