Intracellular metabolite concentrations in glucose-fed, exponentially growing E. coli
Please note: The entry will be shown to all once approved by the database administrator.
| Range | Table - link |
|---|---|
| Organism | Bacteria Escherichia coli |
| Reference | Bennett BD, Kimball EH, Gao M, Osterhout R, Van Dien SJ, Rabinowitz JD. Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli. Nat Chem Biol. 2009 Aug5(8):593-9 p.594 table 1PubMed ID19561621 |
| Method | "[Researchers] quantified metabolites by Liquid chromatography-mass spectrometry (LC-MS/MS) using an isotope ratio-based approach (ref 8). As isotope-labeled standards for many metabolites are not available, they used uniformly 13C-labeled glucose medium to label the intracellular metabolome of E. coli (ref 25). This enabled the use of commercially available unlabeled compounds as internal standards." |
| Comments | "The total observed intracellular metabolite pool was approximately 300 mM (BNID 104678). A small number of metabolites dominate the metabolome on a molar basis, with glutamate being the most abundant." "The absolute cellular concentrations of 103 metabolites were determined in glucose-fed cultures, and most were also determined in glycerol- and acetate-fed cultures (for glucose-grown cells only, see Table 1 for complete data, including error estimates, see Supplementary Table 3)." "[Researchers] used the absolute concentration of metabolites in glucose-grown cultures (Table 1) to assess the extent of saturation of enzymes by their substrates, by comparing the measured concentrations to Km values taken from the BRENDA database (Fig. 2 and Supplementary Table 7)." |
| Entered by | Uri M |
| ID | 104679 |