Intracellular metabolite concentrations in glucose-fed, exponentially growing E. coli
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| Range | Table - link |
|---|---|
| Organism | Bacteria Escherichia coli |
| Reference | Bennett BD, Kimball EH, Gao M, Osterhout R, Van Dien SJ, Rabinowitz JD. Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli. Nat Chem Biol. 2009 Aug5(8):593-9PubMed ID19561621 |
| Method | Researchers quantified metabolites by Liquid chromatography-mass spectrometry (LC-MS/MS) using an isotope ratio-based approach (8). As isotope-labeled standards for many metabolites are not available, they used uniformly 13C-labeled glucose medium to label the intracellular metabolome of E. coli (25). This enabled the use of commercially available unlabeled compounds as internal standards. |
| Comments | The total observed intracellular metabolite pool was approximately 300 mM (BNID 104678). A small number of metabolites dominate the metabolome on a molar basis, with glutamate being the most abundant. |
| Entered by | Uri M |
| ID | 104679 |