EYFP maturation time
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| Value | <=7 min Range: Table - link min |
|---|---|
| Organism | in vitro |
| Reference | Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat Biotechnol. 2002 Jan20(1):87-90 p. 88 table 1PubMed ID11753368 |
| Method | Calculated from Table 1. Based on tau=ln2/(kfold)+ln2/kox where tau is the maturation time, kfold is the folding constant and kox the oxidation constant (the slowest step). To calculate folding rate, the denaturation dynamics was followed and return of fluorescent signal was measured. For oxidation rate, denaturation plus reduction was performed and then renaturation together with dilution of the reducing element and temporal measurement of fluorescence accumulation in vitro. |
| Comments | Value should be ~7 minutes not <= |
| Entered by | Uri M |
| ID | 103780 |