Misincorporation rate of Amino acids in translation
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| Range | 0.001-0.0001 1/amino acid |
|---|---|
| Organism | Bacteria Escherichia coli |
| Reference | Hani S. Zaher and Rachel Green, Fidelity at the Molecular Level: Lessons from Protein Synthesis. Cell Volume 136, Issue 4, 20 February 2009 doi: 10.1016/j.cell.2009.01.036. , Pages 746-762 p.746 left column top paragraphPubMed ID19239893 |
| Primary Source | (1) Bouadloun F, Donner D, Kurland CG., Codon-specific missense errors in vivo, EMBO J. 2 (1983), pp. 1351–1356 AND (2)Edelmann P, Gallant J. Mistranslation in E. coli. Cell. 1977 Jan10(1):131-7PubMed ID10872330, 138485 |
| Method | (Primary source (1) p.1351 right column 3rd paragraph:) "[Researchers'] experimental strategy depends on the ability to identify and purify a polypeptide with a nominal sequence that does not contain the amino acid responsible for the missense substitution under study. As shown in article, the polypeptide can be either a whole protein or an excisable sequence within a whole protein. A second requirement of their method is the availability of a chemical reagent or enzyme that will specifically cleave the polypeptide at the particular amino acid position in the polypeptide at which the putative missense event occurs. Finally, the substituted polypeptide must be resistant to the cleavage reaction. When these conditions are met, a simple combination of radioisotopic measurements of polypeptides fractionated in polyacrylamide gels permits to estimate a specific missense error rate at a particular position in a protein." |
| Comments | "Transcription and translation proceed with considerably lower levels of fidelity, with misincorporation rates of 1 in 10^4 and 1 in 10^3–10^4, respectively [primary sources]." This level of accuracy is lower than that of DNA replication and similar to that of transcription misincorporation rate. See BNID 105069 for LystRNAs misreading rate, BNID 103469 for aminoacylation error rate. See BNID 105718 |
| Entered by | Uri M |
| ID | 103454 |