Size of typical polystrene bead in Tethered Particle Motion method

Range 0.2-1.0 μm
Organism Generic
Reference Garcia HG, Grayson P, Han L, Inamdar M, Kondev J, Nelson PC, Phillips R, Widom J, Wiggins PA. Biological consequences of tightly bent DNA: the other life of a macromolecular celebrity. Biopolymers. 2007 Feb 585(2):115-30.PubMed ID17103419
Method TPM-Individual molecules of a specifically biotinated (RNA in this case) polymerase derivative were tagged an avidin-coated polystyrene beads enzyme movement along a surface-linked DNA molecule was monitored by observing changes in bead Brownian motion by light microscopy.
Entered by Uri M
ID 103167