One dimensional diffusion constant of LacI repressor dimer along DNA segments
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| Value | 0.046 µm^2/sec Range: ±0.01 equivalent to (4.0±0.9)×10^5 bp^2/sec µm^2/sec |
|---|---|
| Organism | Bacteria Escherichia coli |
| Reference | Elf J, Li G, Xie XS. 2007. Probing transcription factor dynamics at the single-molecule level in a living cell. Science 316:1191–94PubMed ID17525339 |
| Method | In vitro single-molecule tracking (of fusion lacI repressor-venus yellow fluorescent protein) on flow-stretched DNA |
| Comments | This is equivalent to D1=(4.0±0.9)×10^5 bp^2/sec (Supporting Online Material pp 5). Note that the apparent diffusion coefficient is one order of magnitude higher, 0.4±0.02µm^2/sec (BNID 102038), as the Lac I dimer spends ~87% of the time nonspecifically bound and diffusing along DNA (D=0.046±0.01µm^2/sec) and the rest of the time unbound to DNA (D=3µm^2/sec). |
| Entered by | Uri M |
| ID | 102036 |