Concentration of dATP in oocyte extracts from Xenopus laevis

Range 5-10 μM
Organism African clawed frog Xenopus laevis
Reference Cox LS, Leno GH. Extracts from eggs and oocytes of Xenopus laevis differ in their capacities for nuclear assembly and DNA replication. J Cell Sci. 1990 Sep97 (Pt 1):177-84. p.178 right column 2nd paragraphPubMed ID2258387
Method isotope dilution analysis
Comments P.178 right column 2nd paragraph: "Oocyte extracts have been shown by isotope dilution analysis to contain 5-10µM dATP (L.S.C., unpublished results), hence all oocyte extracts were supplemented with 45µM dNTPs to bring precursor pools up to the levels reported in egg extracts (i.e. 50µM: dATP, Blow and Laskey, 1986, dCTP, Hutchison et al. 1987)."
Entered by Paul Jorgensen
ID 101451