Maximal emission wavelength for dsRed fluorophore

Value 583 nm
Organism Discosoma striata
Reference Patterson G, Day RN, Piston D. Fluorescent protein spectra, J Cell Sci. 2001 Mar114(Pt 5):837-8. p.838 table 1 link - link PubMed ID11181166
Primary Source Matz MV, Fradkov AF, Labas YA, Savitsky AP, Zaraisky AG, Markelov ML, Lukyanov SA. Fluorescent proteins from nonbioluminescent Anthozoa species. Nat Biotechnol. 1999 Oct17(10):969-73. table 2PubMed ID10504696
Method (Primary source) Proteins were expressed in E. coli with His6 tags at their N termini and purified using TALON metal affinity resin (Clontech). Proteins were at least 95% pure according to SDS–PAGE. Quantum yields for novel proteins were determined relative to wild-type GFP (Clontech). Perkin-Elmer LS50B spectrometer (Beaconsfield, UK) was used for quantitative measurements. All samples were excited at 470 nm, absorbance at this wavelength was 0.02, and excitation and emission slits were 5 nm. The spectra were corrected for photomultiplier response and monochromator transmittance, transformed to wave number and integrated.
Comments Protein originally from Discosoma striata, several rounds of optimziations via mutations were performed. The recent cloning of a gene that encodes a red fluorescent protein (dsRed) from the Indo-Pacific sea anemone Discosoma striata has provided yet another fluorescent protein that is further red-shifted (primary source).
Entered by Cellina Cohen-Saidon
ID 101095