Genome Size
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| Value | 4.6e+6 Base pairs |
|---|---|
| Organism | Bacteria Escherichia coli |
| Reference | Blattner FR et al, The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5 277(5331):1453-74.PubMed ID9278503 |
| Method | Sequencing was carried out in sections, with steadily improving technical approaches. The M13 Janus shotgun strategy proved to be the most efficient strategy for data collection and closure. It involved initial random sequencing at a four- to fivefold redundancy in the Janus vector (Burland et al 1993, PMID 8346017), followed by computerized selection of templates to be resequenced from the opposite end, followed by limited primer walking (p. 1453). |
| Comments | The genome of E. coli K-12 consists of 4,639,221 bp of circular duplex DNA (p. 1454) (i.e., a single chromosome). |
| Entered by | Ron Milo - Admin |
| ID | 100269 |